Development of a biosafety level-2 facility for irradiation of biological cells using MeV ions

Authors

Jay D. Diaz, University of Louisiana at Lafayette
Naresh T. Deoli, University of Louisiana at Lafayette
Deborah Rogers, University of Louisiana at Lafayette
Armin de Vera, University of Louisiana at Lafayette
Karen M. Smith, University of Louisiana at Lafayette
Robin Schmeider-Broussard, University of Louisiana at Lafayette
Harry J. Whitlow, University of Louisiana at Lafayette

Document Type

Article

Abstract

The development of a facility for irradiation of murice and primate tissue presents several challenges. First, extremely low fluences (10⁶-10¹¹ ions cm⁻²) are required to deliver doses of 1 mGy - 50 Gy using MeV proton. Second,the tissues, particularly from non-human primate, may be infectious to humans. Third, protection of irradiated tissue samples from contamination by mold and bacteria is critical. Here we present an ultra-low flux irradiation facility and associated fluence control for biological cell and tissue irradiation under sterile biosafety level 2 (BSL-2) conditions suitable for use with low energy (1-3.4 MeV) protons. The operation is demonstrated using murine astocytes.

DOI

https://doi.org/10.1016/j.nimb.2019.03.002

Publication Date

2019

Recommended Citation

Diaz, Jay D.; Deoli, Naresh T.; Rogers, Deborah; de Vera, Armin; Smith, Karen M.; Schmeider-Broussard, Robin; and Whitlow, Harry J., "Development of a biosafety level-2 facility for irradiation of biological cells using MeV ions" (2019). Louisiana Accelerator Center. 1.
https://scholarshub.louisiana.edu/lac/1